Sandwich ELISA with human plasma using recombinant Human IgG2 antibody as the capture, and biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129 as the detect, followed by an AP conjugated streptavidin.
Sandwich ELISA with human IgG2 using recombinant Human IgG2 antibody as the capture, and biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129 as the detect, followed by an AP conjugated streptavidin.
ELISA of human immunoglobulins shows recombinant Human IgG2 antibody only reacted to hIgG2. No cross reactivity with IgG1, IgG3, IgG4, IgE, IgD, IgA, mouse/rat/goat IgG.
ELISA Titration: the plate was coated with different amounts of human IgG2. A serial dilution of recombinant Human IgG2 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Supplier Page from NSJ Bioreagents for Human IgG2 Antibody [Recombinant Rabbit Monoclonal]